Amplification Biochips and Implantable Bar-code Sensors

Thrust Leads

Goals: Thrust 1 will develop amplification biochips and implantable bar-code sensors for point-of-care (POC) monitoring of cardiovascular disease and diabetes biomarkers. There are two main projects for Thrust 1.

Project 1.1: Design and validation of cartridge-based assays and systems using microfluidics, paper fluidics, nanoparticles, and amplification

Project 1.1 is designed to be integrated with T2.1, namely the design and validation of mobile and cost-effective reader platforms to overcome key challenges and gaps in existing technologies toward developing the Lab in your Palm-engineered systems for cardiovascular and diabetes applications.

This project includes three tasks focused on the parallel development of (1) nanostructure-based biosensors, (2) cost-effective material-based biosensors, and (3) emerging biomarkers technologies.

Dino DiCarlo

UCLA Thrust Co-Lead

Gerard L. Coté

TAMU

Jessica Ramella-Roman

FIU

Aydogan Ozcan

UCLA

Samuel Mabbott

TAMU

Josh Hutcheson

FIU

Omai Garner

UCLA

Carl Tong

TAMU

Paper-based multi target diagnostic test enables new applications

Thrust 1.1 Research Highlights

Lab on a Chip (2021)- Front Cover

Counting of enzymatically amplified affinity reactions in hydrogel particle-templated drops – Lab on a Chip (RSC Publishing)

Yilian Wang, Vishwesh Shah, Angela Lu, Ella Pachler, Brian Cheng, Dino Di Carlo

ACS Sensors (2020) – Front Cover

Fabrication of 3D concentric amphiphilic microparticles to form uniform nanoliter reaction volumes for amplified affinity assays

Ghulam Destgeer, Mengxing Ouyang, Chueh-Yu Wua and Dino Di Carlo

ACS Sensors (2021) – Editors Choice

Methylation-Sensitive Loop-Mediated Isothermal Amplification (LAMP): Nucleic Acid Methylation Detection through LAMP with Mobile Fluorescence Readout

Jacob Amos Hambalek, Janay Elise Kong, Calvin Brown, Hector Enrique Munoz, Thomas Horn, Michael Bogumil, Eleni Quick, Aydogan Ozcan, and Dino Di Carlo

Project 1.2: Design and validation of implantable systems including optimization of the transduction and recognition elements of the assay and development of biocompatible materials

T1.2 is designed to be integrated with T2 project 2 and T3 projects 1 and 3, to overcome the key challenges of the Lab on your Wrist implantable engineered system primarily for diabetes applications.

This project includes two main tasks focused on the parallel development of (1) biocompatible materials for encapsulating/packaging bio-sensing assays, and (2) multiplexed (“barcoded”) assays for monitoring multiple analytes with a single inserted device.